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Complete sequence of a bla(KPC)-harboring cointegrate plasmid isolated from Escherichia coli.

ABSTRACT: Horizontal transfer of bla(KPC)-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla(KPC)-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how bla(KPC) can be spread from a nonconjugative plasmid through cointegration.

SUBMITTER: Chavda KD

PROVIDER: S-EPMC4394832 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Complete sequence of a bla(KPC)-harboring cointegrate plasmid isolated from Escherichia coli.

Chavda Kalyan D KD Chen Liang L Jacobs Michael R MR Rojtman Albert D AD Bonomo Robert A RA Kreiswirth Barry N BN

Antimicrobial agents and chemotherapy 20150309 5

Horizontal transfer of bla(KPC)-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla(KPC)-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstr ...[more]

PMID: 25753632

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