Dataset Information

First Report of Complete Sequence of a blaNDM-13-Harboring Plasmid from an Escherichia coli ST5138 Clinical Isolate.

ABSTRACT: Since the first report of blaNDM-1, 16 blaNDM variants have been identified among Gram-negative bacteria worldwide. Recently, a novel blaNDM variant, blaNDM-13, was identified in the chromosome of an ST101 Escherichia coli isolate from Nepal. Here we first reported plasmid-mediated blaNDM-13 in a carbapenem-resistant E. coli ST5138 clinical isolate associated with hospital-acquired urinary tract infection from China. blaNDM-13 and blaSHV-12 coexisted on the a ~54 Kb self-transferable plasmid. Compared with NDM-1, NDM-13, NDM-3, and NDM-4 had two amino acid substitutions (D95N and M154L), one amino acid substitution (D95N) and one amino acid substitutions (M154L), respectively. Complete plasmid sequencing showed that blaNDM-13-harboring plasmid (pNDM13-DC33) was highly similar to the blaNDM-1-harboring IncX3 plasmid pNDM-HN380, a common blaNDM-harboring vector circulating in China. In accordance with the structure of pNDM-HN380, pNDM13-DC33 consists of a 33-kb backbone encoding plasmid replication (repB), stability partitioning, and transfer (tra, trb, and pil) functions, and a 21-kb antimicrobial resistance region with high GC content between umuD and mpr genes. In conclusion, the present study is the first report of a plasmid-encoded blaNDM-13 and the complete sequence of a blaNDM-13-harboring plasmid (pNDM13-DC33). blaNDM-13 maybe originate from blaNDM-1 located on a pNDM-HN380-like plasmid by sequential mutations.

SUBMITTER: Lv J

PROVIDER: S-EPMC5061728 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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First Report of Complete Sequence of a blaNDM-13-Harboring Plasmid from an Escherichia coli ST5138 Clinical Isolate.

Lv Jingnan J Qi Xiuqin X Zhang Dan D Zheng Zhou Z Chen Yuehui Y Guo Yinjuan Y Wang Shanshan S Chen Liang L Kreiswirth Barry N BN Tang Yi-Wei YW Chen Zengqiang Z Hu Longhua L Wang Liangxing L Yu Fangyou F

Frontiers in cellular and infection microbiology 20161013

Since the first report of blaNDM-1, 16 blaNDM variants have been identified among Gram-negative bacteria worldwide. Recently, a novel blaNDM variant, blaNDM-13, was identified in the chromosome of an ST101 Escherichia coli isolate from Nepal. Here we first reported plasmid-mediated blaNDM-13 in a carbapenem-resistant E. coli ST5138 clinical isolate associated with hospital-acquired urinary tra ...[more]

PMID: 27790412

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Project description:The plasmid-mediated transmission of antibiotic resistance genes has been reported to be involved in the development of antibiotic resistance in bacteria, and poses a serious threat for the success of bacterial infection treatment and human health worldwide. The present study used a 454 GS?FLX pyrosequencing system to determine the ~140 kb nucleotide sequence of plasmid pHS36?NDM, which was identified in a Salmonella Stanley isolate from the stool sample of an 11?month?old girl at Lishui Central Hospital, China, and which contains a New Delhi metallo???lactamase?1 (NDM?1) carbapenem resistance gene (blaNDM?1). The 181 open reading frames encode proteins with functions including replication, stable inheritance, antibiotic resistance and mobile genetic elements. Both horizontal transfer and passage stability?related genes were identified in pHS36?NDM, including a conserved type 4 secretion system and stbA (stable plasmid inheritance protein A). Two multidrug resistance gene islands were identified: The ISEcp1?blaCMY transposition unit which contains a CMY?6 ??lactamase gene (blaCMY?6) and a quaternary ammonium compound resistance gene (sugE); and the intI1?ISCR27 accessory region, which contained a trimethoprim resistance gene (dfrA12), two aminoglycoside resistance genes (aadA2 and rmtC), a truncated quaternary ammonium compound resistance gene (qacE?1), a sulfonamide resistance gene (sul1), the blaNDM?1 carbapenemase and a bleomycin resistance gene (bleMBL). pHS36?NDM shared high homology with other blaNDM?1?containing plasmids reported in Sweden, Italy and Japan. However, no previous international travel history was documented for the patient and her family, even to neighboring cities. Furthermore, pHS36?NDM is of a different incompatibility group to other published blaNDM?1?carrying plasmids reported in China, with low homology in the surrounding structure of blaNDM?1. The present study will facilitate the understanding of the underlying resistance and dispersal mechanism of pHS36?NDM, and will deepen our recognition of the ongoing spread of the blaNDM?1?containing plasmids.

Dataset Information

First Report of Complete Sequence of a blaNDM-13-Harboring Plasmid from an Escherichia coli ST5138 Clinical Isolate.

Publications

First Report of Complete Sequence of a <i>bla</i><sub>NDM-13</sub>-Harboring Plasmid from an <i>Escherichia coli</i> ST5138 Clinical Isolate.

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