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Ex vivo cytosolic delivery of functional macromolecules to immune cells.

ABSTRACT: Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.

SUBMITTER: Sharei A

PROVIDER: S-EPMC4395260 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Ex vivo cytosolic delivery of functional macromolecules to immune cells.

Sharei Armon A Trifonova Radiana R Jhunjhunwala Siddharth S Hartoularos George C GC Eyerman Alexandra T AT Lytton-Jean Abigail A Angin Mathieu M Sharma Siddhartha S Poceviciute Roberta R Mao Shirley S Heimann Megan M Liu Sophia S Talkar Tanya T Khan Omar F OF Addo Marylyn M von Andrian Ulrich H UH Anderson Daniel G DG Langer Robert R Lieberman Judy J Jensen Klavs F KF

PloS one 20150413 4

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated i ...[more]

PMID: 25875117

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