Dataset Information

Nicotinamide exacerbates hypoxemia in ventilator-induced lung injury independent of neutrophil infiltration.

ABSTRACT:

Background

Ventilator-induced lung injury is a form of acute lung injury that develops in critically ill patients on mechanical ventilation and has a high degree of mortality. Nicotinamide phosphoribosyltransferase is an enzyme that is highly upregulated in ventilator-induced lung injury and exacerbates the injury when given exogenously. Nicotinamide (vitamin B3) directly inhibits downstream pathways activated by Nicotinamide phosphoribosyltransferase and is protective in other models of acute lung injury.

Methods

We administered nicotinamide i.p. to mice undergoing mechanical ventilation with high tidal volumes to study the effects of nicotinamide on ventilator-induced lung injury. Measures of injury included oxygen saturations and bronchoalveolar lavage neutrophil counts, protein, and cytokine levels. We also measured expression of nicotinamide phosophoribosyltransferase, and its downstream effectors Sirt1 and Cebpa, Cebpb, Cebpe. We assessed the effect of nicotinamide on the production of nitric oxide during ventilator-induced lung injury. We also studied the effects of ventilator-induced lung injury in mice deficient in C/EBP?.

Results

Nicotinamide treatment significantly inhibited neutrophil infiltration into the lungs during ventilator-induced lung injury, but did not affect protein leakage or cytokine production. Surprisingly, mice treated with nicotinamide developed significantly worse hypoxemia during mechanical ventilation. This effect was not linked to increases in nitric oxide production or alterations in expression of Nicotinamide phosphoribosyl transferase, Sirt1, or Cebpa and Cebpb. Cebpe mRNA levels were decreased with either nicotinamide treatment or mechanical ventilation, but mice lacking C/EBP? developed the same degree of hypoxemia and ventilator-induced lung injury as wild-type mice.

Conclusions

Nicotinamide treatment during VILI inhibits neutrophil infiltration of the lungs consistent with a strong anti-inflammatory effect, but paradoxically also leads to the development of significant hypoxemia. These findings suggest that pulmonary neutrophilia is not linked to hypoxemia in ventilator-induced lung injury, and that nicotinamide exacerbates hypoxemia during VILI.

SUBMITTER: Jones HD

PROVIDER: S-EPMC4395431 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Nicotinamide exacerbates hypoxemia in ventilator-induced lung injury independent of neutrophil infiltration.

Jones Heather D HD Yoo Jeena J Crother Timothy R TR Kyme Pierre P Ben-Shlomo Anat A Khalafi Ramtin R Tseng Ching W CW Parks William C WC Arditi Moshe M Liu George Y GY Shimada Kenichi K

PloS one 20150413 4

<h4>Background</h4>Ventilator-induced lung injury is a form of acute lung injury that develops in critically ill patients on mechanical ventilation and has a high degree of mortality. Nicotinamide phosphoribosyltransferase is an enzyme that is highly upregulated in ventilator-induced lung injury and exacerbates the injury when given exogenously. Nicotinamide (vitamin B3) directly inhibits downstream pathways activated by Nicotinamide phosphoribosyltransferase and is protective in other models of ...[more]

PMID: 25875775

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Project description:Binge drinking among alcohol consumers is a common occurrence, and may result in the development of numerous diseases, including liver disorders. It has previously been reported that natural killer T (NKT) cells induce alcohol?associated liver injury by promoting neutrophil infiltration. In the present study, the role of the orphan nuclear receptor small heterodimer partner (SHP), which is encoded by the NR0B2 gene, in acute binge drinking?induced liver injury was investigated. SHP?knockout (KO) and wild?type (WT) control mice were intragastrically administered single doses of alcohol. The plasma concentrations of alanine aminotransferase and aspartate aminotransferase in SHP?KO mice following alcohol treatment were significantly increased compared with WT mice. However, results of oil red O staining and 2',7'?dichlorodihydrofluorescein diacetate staining indicated that levels of acute binge drinking?associated hepatic lipid accumulation and oxidative stress were not significantly different between WT and SHP?KO alcohol?treated mice. Notably, tumor necrosis factor?? mRNA expression in the liver of SHP?KO mice was significantly increased following alcohol administration, compared with WT mice. Furthermore, the mRNA expression levels of C?C motif chemokine ligand 2, C?X?C motif chemokine ligand 2 and interleukin?4, which are all potent chemoattractants of NKT cells, as well as neutrophil expression levels, were significantly increased in the livers of SHP?KO mice compared with WT mice following alcohol administration, as determined by reverse transcription?quantitative polymerase chain reaction and flow cytometry. Enhanced infiltration of NKT cells, determined by flow cytometry, was also demonstrated in the livers of SHP?KO mice following alcohol administration, compared with WT mice. The results of the present study indicate that SHP may be involved in liver?associated protective mechanisms, with regards to the attenuation of damage caused by acute binge drinking, via regulation of NKT cell and neutrophil migration to the liver. The modulation of SHP may be a novel therapeutic strategy for the treatment of acute binge drinking?induced liver injury.

Dataset Information

Nicotinamide exacerbates hypoxemia in ventilator-induced lung injury independent of neutrophil infiltration.

Background

Methods

Results

Conclusions

Publications

Nicotinamide exacerbates hypoxemia in ventilator-induced lung injury independent of neutrophil infiltration.

Similar Datasets

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