Ontology highlight
ABSTRACT:
SUBMITTER: Calvino FR
PROVIDER: S-EPMC4396368 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Calviño Fabiola R FR Kharde Satyavati S Ori Alessandro A Hendricks Astrid A Wild Klemens K Kressler Dieter D Bange Gert G Hurt Ed E Beck Martin M Sinning Irmgard I
Nature communications 20150407
During 60S biogenesis, mature 5S RNP consisting of 5S RNA, RpL5 and RpL11, assembles into a pre-60S particle, where docking relies on RpL11 interacting with helix 84 (H84) of the 25S RNA. How 5S RNP is assembled for recruitment into the pre-60S is not known. Here we report the crystal structure of a ternary symportin Syo1-RpL5-N-RpL11 complex and provide biochemical and structural insights into 5S RNP assembly. Syo1 guards the 25S RNA-binding surface on RpL11 and competes with H84 for binding. P ...[more]