Ontology highlight
ABSTRACT:
SUBMITTER: Bartoszewska S
PROVIDER: S-EPMC4396612 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Bartoszewska Sylwia S Kochan Kinga K Piotrowski Arkadiusz A Kamysz Wojciech W Ochocka Renata J RJ Collawn James F JF Bartoszewski Rafal R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20141230 4
Hypoxia-inducible factors (HIFs) 1 and 2 are dimeric α/β transcription factors that regulate cellular responses to low oxygen. HIF-1 is induced first, whereas HIF-2 is associated with chronic hypoxia. To determine how HIF1A mRNA, the inducible subunit of HIF-1, is regulated during hypoxia, we followed HIF1A mRNA levels in primary HUVECs over 24 hours using quantitative PCR. HIF1A and VEGF A (VEGFA) mRNA, a transcriptional target of HIF-1, increased ∼ 2.5- and 8-fold at 2-4 hours, respectively. T ...[more]