Dataset Information
Overexpression of the novel senescence marker ?-galactosidase (GLB1) in prostate cancer predicts reduced PSA recurrence.
Ontology highlight
ABSTRACT: Purpose
Senescence is a terminal growth arrest that functions as a tumor suppressor in aging and precancerous cells and is a response to selected anticancer compounds. Lysosomal-?-galactosidase (GLB1) hydrolyzes ?-galactose from glycoconjugates and is the origin of senescence-associated ?-gal activity (SA-?-gal). Using a new GLB1 antibody, senescence biology was investigated in prostate cancer (PCa) tissues.Experimental design
In vitro characterization of GLB1 was determined in primary prostate epithelial cell cultures passaged to replicative senescence and in therapy-induced senescence in PCa lines using chemotherapeutic agents. FFPE tissue microarrays were subjected to immunofluorescent staining for GLB1, Ki67 and HP1? and automated quantitative imaging initially using AQUA in exploratory samples and Vectra in a validation series.Results
GLB1 expression accumulates in replicative and induced senescence and correlates with senescent morphology and P16 (CDKN2) expression. In tissue arrays, quantitative imaging detects increased GLB1 expression in high-grade prostatic intraepithelial neoplasia (HGPIN), known to contain senescent cells, and cancer compared to benign prostate tissues (p<0.01) and senescent cells contain low Ki67 and elevated HP1?. Within primary tumors, elevated GLB1 associates with lower T stage (p=0.01), localized versus metastatic disease (p=0.0003) and improved PSA-free survival (p=0.03). Increased GLB1 stratifies better PSA-free survival in intermediate grade PCa (0.01). Tissues that elaborate higher GLB1 display increased uniformity of expression.Conclusion
Increased GLB1 is a valuable marker in formalin-fixed paraffin-embedded (FFPE) tissues for the senescence-like phenotype and associates with improved cancer outcomes. This protein addresses a lack of senescence markers and should be applicable to study the biologic role of senescence in other cancers.
SUBMITTER: Wagner J
PROVIDER: S-EPMC4398352 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Overexpression of the novel senescence marker β-galactosidase (GLB1) in prostate cancer predicts reduced PSA recurrence.
PloS one 20150415 4
<h4>Purpose</h4>Senescence is a terminal growth arrest that functions as a tumor suppressor in aging and precancerous cells and is a response to selected anticancer compounds. Lysosomal-β-galactosidase (GLB1) hydrolyzes β-galactose from glycoconjugates and is the origin of senescence-associated β-gal activity (SA-β-gal). Using a new GLB1 antibody, senescence biology was investigated in prostate cancer (PCa) tissues.<h4>Experimental design</h4>In vitro characterization of GLB1 was determined in p ...[more]