Ontology highlight
ABSTRACT:
SUBMITTER: Morin JA
PROVIDER: S-EPMC4402526 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Nucleic acids research 20150323 7
During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers to study the translocation mechanism of individual bacteriophage Phi29 DNA polymerases during processive DNA replication. We determine the main kinetic parameters of the nucleotide incorporation cycle and their dependence on external load and nucleotide (dNTP) concentration. The data ...[more]