Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
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ABSTRACT:
SUBMITTER: Yuan M
PROVIDER: S-EPMC4403460 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Publications
Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
Yuan Ming M Zhang Wensheng W Wang Jun J Al Yaghchi Chadwan C Ahmed Jahangir J Chard Louisa L Lemoine Nick R NR Wang Yaohe Y
Journal of virology 20150304 9
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV ...[more]
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