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Inducible and combinatorial gene manipulation in mouse brain.

ABSTRACT: We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate 'when', 'where', and 'how' gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

SUBMITTER: Dogbevia GK

PROVIDER: S-EPMC4404871 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Inducible and combinatorial gene manipulation in mouse brain.

Dogbevia Godwin K GK Marticorena-Alvarez Ricardo R Bausen Melanie M Sprengel Rolf R Hasan Mazahir T MT

Frontiers in cellular neuroscience 20150421

We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate 'when', 'where', and 'how' gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, ...[more]

PMID: 25954155

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