Voltage controlled nano-injection system for single-cell surgery.

ABSTRACT: Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes.

SUBMITTER: Adam Seger R 

PROVIDER: S-EPMC4406976 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature