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Blending of diblock and triblock copolypeptide amphiphiles yields cell penetrating vesicles with low toxicity.

ABSTRACT: We prepared dual hydrophilic triblock copolypeptide vesicles that form both micron and nanometer scale vesicles in aqueous media. The incorporation of terminal homoarginine segments into methionine sulfoxide-based vesicles was found to significantly enhance their cellular uptake compared to a non-ionic control. We also demonstrated that diblock and triblock copolypeptides with similar hydrophobic domains were found to mix well and form vesicle populations with uniform compositions. Blending of amphiphiles in vesicle nanocarriers was found to impart these materials with many advantageous properties, including good cellular uptake while maintaining minimal toxicity, as well as biological responsiveness to promote vesicle disruption and release of encapsulated cargos.

SUBMITTER: Rodriguez AR

PROVIDER: S-EPMC4408078 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Blending of diblock and triblock copolypeptide amphiphiles yields cell penetrating vesicles with low toxicity.

Rodriguez April R AR Choe Uh-Joo UJ Kamei Daniel T DT Deming Timothy J TJ

Macromolecular bioscience 20141016 1

We prepared dual hydrophilic triblock copolypeptide vesicles that form both micron and nanometer scale vesicles in aqueous media. The incorporation of terminal homoarginine segments into methionine sulfoxide-based vesicles was found to significantly enhance their cellular uptake compared to a non-ionic control. We also demonstrated that diblock and triblock copolypeptides with similar hydrophobic domains were found to mix well and form vesicle populations with uniform compositions. Blending of a ...[more]

PMID: 25323924

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