Unknown

Dataset Information

0

Inflammation triggers specific microRNA profiles in human adipocytes and macrophages and in their supernatants.


ABSTRACT: The relevance of microRNAs (miRNAs) in adipose tissue is increasingly recognized, being intrinsically linked to different pathways, including obesity-related inflammation. In this study, we aimed to characterize the changes induced by inflammation on the miRNA pattern of human adipocytes and macrophages. Therefore, an extensive profile of 754 common miRNAs was assessed in cells (human primary mature adipocytes, and the macrophage-like cell line THP-1) and in their supernatants (SN) using TaqMan low-density arrays. These profiles were evaluated at the baseline and after administration of lipopolysaccharide (LPS, 10 ng/ml) and LPS-conditioned medium from M1 macrophages (MCM, 5%). The miRNAs that experienced the most dramatic changes were studied in subcutaneous human adipose tissue before and approximately 2 years after bariatric surgery-induced weight loss.Differentiated adipocytes expressed 169 miRNAs, being 85 detectable in the SN. In M1 macrophages, 183 miRNAs were detected, being 106 also present in the SN. Inflammation led to an increased number of miRNAs detectable in cells and in their SNs in both adipocytes (+8.3% and +24.7%) and M1 macrophages (+1.4% and +5%, respectively). Indeed, under inflammatory conditions, adipocytes and M1 macrophages shared the expression of 147 (+9%) miRNAs, and 100 (+41%) common miRNAs were found in their SNs. Twelve of these factors were also linked to inflammation in whole adipose tissue from obese subjects. Interestingly, miR-221 (2-fold, P?=?0.002), miR-222 (2.5-fold, P?=?0.04), and miR-155 (5-fold, P?=?0.015) were increased in inflamed adipocytes and in their SNs (15-, 6-, and 4-fold, respectively, all P?

SUBMITTER: Ortega FJ 

PROVIDER: S-EPMC4413548 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Inflammation triggers specific microRNA profiles in human adipocytes and macrophages and in their supernatants.

Ortega Francisco José FJ   Moreno María M   Mercader Josep María JM   Moreno-Navarrete José María JM   Fuentes-Batllevell Núria N   Sabater Mònica M   Ricart Wifredo W   Fernández-Real José Manuel JM  

Clinical epigenetics 20150424


<h4>Background</h4>The relevance of microRNAs (miRNAs) in adipose tissue is increasingly recognized, being intrinsically linked to different pathways, including obesity-related inflammation. In this study, we aimed to characterize the changes induced by inflammation on the miRNA pattern of human adipocytes and macrophages. Therefore, an extensive profile of 754 common miRNAs was assessed in cells (human primary mature adipocytes, and the macrophage-like cell line THP-1) and in their supernatants  ...[more]

Similar Datasets

| S-EPMC3063154 | biostudies-literature
| S-EPMC6985838 | biostudies-literature
| S-EPMC6947878 | biostudies-literature
| S-EPMC5083931 | biostudies-literature
| S-EPMC6370809 | biostudies-literature
| S-EPMC2422824 | biostudies-literature
| S-EPMC6215669 | biostudies-literature
| S-EPMC4410449 | biostudies-literature
| S-EPMC7865382 | biostudies-literature
| S-EPMC2800214 | biostudies-literature