Sequencing small genomic targets with high efficiency and extreme accuracy.
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ABSTRACT:
SUBMITTER: Schmitt MW
PROVIDER: S-EPMC4414912 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Publications
Sequencing small genomic targets with high efficiency and extreme accuracy.
Schmitt Michael W MW Fox Edward J EJ Prindle Marc J MJ Reid-Bayliss Kate S KS True Lawrence D LD Radich Jerald P JP Loeb Lawrence A LA
Nature methods 20150406 5
The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules. ...[more]
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