Dataset Information
Development of a reporter peptide that catalytically produces a fluorescent signal through ?-complementation.
Ontology highlight
ABSTRACT:
SUBMITTER: Nishiyama K
PROVIDER: S-EPMC4420511 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Publications
Development of a reporter peptide that catalytically produces a fluorescent signal through α-complementation.
Protein science : a publication of the Protein Society 20150402 5
In α-complementation, inactive N-terminal (α-domain) and C-terminal (ω-domain) fragments of β-galactosidase associate to reconstitute the active protein. To date, the effect of α-domain size on α-complementation activity has not been systematically investigated. In this study, we compared the complementation activities of α-domains of various sizes using an in vitro system. We found that the complementation activities are similar for α-domains comprising between 45 and 229 N-terminal residues bu ...[more]
