Transforming growth factor-?1 regulated phosphorylated AKT and interferon gamma expressions are associated with epithelial cell survival in rhesus macaque colon explants.

ABSTRACT: Transforming growth factor-?1 (TGF-?1) is an important immunoregulatory cytokine that plays an obligate role in regulating T-cell functions. Here, we demonstrated the role of TGF-?1 in regulating the survival of intestinal epithelial cells (ECs) in rhesus colon explant cultures using either anti-TGF-?1 antibody or recombinant TGF-?1 proteins. Neutralization of endogenous TGF-?1 using anti-TGF-?1 antibodies induced apoptosis of both intestinal ECs and lamina propria (LP) cells. Additionally, endogenous TGF-?1 blocking significantly increased expression of IFN?, TNF?, CD107a and Perforin in LP cells compared to media and isotype controls. A significant decrease in pAKT expression was detected in anti-TGF-?1 MAbs treated explants compared to isotype and rTGF-?1 protein treated explants. Our results demonstrated TGF-?1 regulated pAKT and IFN? expressions were associated with epithelial cell survival in rhesus macaque colon explants and suggest a potential role of mucosal TGF-?1 in regulating intestinal homeostasis and EC integrity.

SUBMITTER: Pahar B 

PROVIDER: S-EPMC4420722 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature