Dataset Information

Electrical stimulation improves microbial salinity resistance and organofluorine removal in bioelectrochemical systems.

ABSTRACT: Fed batch bioelectrochemical systems (BESs) based on electrical stimulation were used to treat p-fluoronitrobenzene (p-FNB) wastewater at high salinities. At a NaCl concentration of 40 g/liter, p-FNB was removed 100% in 96 h in the BES, whereas in the biotic control (BC) (absence of current), p-FNB removal was only 10%. By increasing NaCl concentrations from 0 g/liter to 40 g/liter, defluorination efficiency decreased around 40% in the BES, and in the BC it was completely ceased. p-FNB was mineralized by 30% in the BES and hardly in the BC. Microorganisms were able to store 3.8 and 0.7 times more K(+) and Na(+) intracellularly in the BES than in the BC. Following the same trend, the ratio of protein to soluble polysaccharide increased from 3.1 to 7.8 as the NaCl increased from 0 to 40 g/liter. Both trends raise speculation that an electrical stimulation drives microbial preference toward K(+) and protein accumulation to tolerate salinity. These findings are in accordance with an enrichment of halophilic organisms in the BES. Halobacterium dominated in the BES by 56.8% at a NaCl concentration of 40 g/liter, while its abundance was found as low as 17.5% in the BC. These findings propose a new method of electrical stimulation to improve microbial salinity resistance.

SUBMITTER: Feng H

PROVIDER: S-EPMC4421048 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Electrical stimulation improves microbial salinity resistance and organofluorine removal in bioelectrochemical systems.

Feng Huajun H Zhang Xueqin X Guo Kun K Vaiopoulou Eleni E Shen Dongsheng D Long Yuyang Y Yin Jun J Wang Meizhen M

Applied and environmental microbiology 20150327 11

Fed batch bioelectrochemical systems (BESs) based on electrical stimulation were used to treat p-fluoronitrobenzene (p-FNB) wastewater at high salinities. At a NaCl concentration of 40 g/liter, p-FNB was removed 100% in 96 h in the BES, whereas in the biotic control (BC) (absence of current), p-FNB removal was only 10%. By increasing NaCl concentrations from 0 g/liter to 40 g/liter, defluorination efficiency decreased around 40% in the BES, and in the BC it was completely ceased. p-FNB was miner ...[more]

PMID: 25819966

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Project description:BackgroundSoil microbial fuel cells (MFCs) can remove antibiotics and antibiotic resistance genes (ARGs) simultaneously, but their removal mechanism is unclear. In this study, metagenomic analysis was employed to reveal the functional genes involved in degradation, electron transfer and the nitrogen cycle in the soil MFC.ResultsThe results showed that the soil MFC effectively removed tetracycline in the overlapping area of the cathode and anode, which was 64% higher than that of the control. The ARGs abundance increased by 14% after tetracycline was added (54% of the amplified ARGs belonged to efflux pump genes), while the abundance decreased by 17% in the soil MFC. Five potential degraders of tetracycline were identified, especially the species Phenylobacterium zucineum, which could secrete the 4-hydroxyacetophenone monooxygenase encoded by EC 1.14.13.84 to catalyse deacylation or decarboxylation. Bacillus, Geobacter, Anaerolinea, Gemmatirosa kalamazoonesis and Steroidobacter denitrificans since ubiquinone reductase (encoded by EC 1.6.5.3), succinate dehydrogenase (EC 1.3.5.1), Coenzyme Q-cytochrome c reductase (EC 1.10.2.2), cytochrome-c oxidase (EC 1.9.3.1) and electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) served as complexes I, II, III, IV and ubiquinone, respectively, to accelerate electron transfer. Additionally, nitrogen metabolism-related gene abundance increased by 16% to support the microbial efficacy in the soil MFC, and especially EC 1.7.5.1, and coding the mutual conversion between nitrite and nitrate was obviously improved.ConclusionsThe soil MFC promoted functional bacterial growth, increased functional gene abundance (including nitrogen cycling, electron transfer, and biodegradation), and facilitated antibiotic and ARG removal. Therefore, soil MFCs have expansive prospects in the remediation of antibiotic-contaminated soil. This study provides insight into the biodegradation mechanism at the gene level in soil bioelectrochemical remediation.

Project description:Bioremediation of polychlorinated biphenyls (PCBs) has been precluded in part by the lack of a cost-effective method to stimulate microbial degradation in situ. A common limitation is the lack of an effective method of providing electron donors and acceptors to promote in situ PCB biodegradation. Application of an electric potential to soil/sediment could be an effective means of providing electron-donors/-acceptors to PCB dechlorinating and degrading microorganisms. In this study, electrical stimulation of microbial PCB dechlorination/degradation was examined in sediment maintained under simulated in situ conditions. Voltage was applied to open microcosms filled with PCB-impacted (Aroclor 1242) freshwater sediment from a Superfund site (Fox River, WI). The effect of applied low voltages (1.5-3.0 V) on the microbial transformation of PCBs was determined with: 1) spiked PCBs, and 2) indigenous weathered PCBs. The results indicate that both oxidative and reductive microbial transformation of the spiked PCBs was stimulated but oxidation was dominant and most effective with higher voltage. Chlorobenzoates were produced as oxidation metabolites of the spiked PCBs, but increasing voltage enhanced chlorobenzoate consumption, indicating that overall degradation was enhanced. In the case of weathered PCBs, the total concentration decreased 40-60% in microcosms exposed to electric current while no significant decrease of PCB concentration was observed in control reactors (0 V or sterilized). Single congener analysis of the weathered PCBs showed significant loss of di- to penta-chlorinated congeners, indicating that microbial activity was not limited to anaerobic dechlorination of only higher chlorinated congeners. Degradation was most apparent with the application of only 1.5 V where anodic O(2) was not generated, indicating a mechanism of degradation independent of electrolytic O(2). Low voltage stimulation of the microbial degradation of weathered PCBs observed in this study suggests that this approach could be a cost-effective, environmentally sustainable strategy to remediate PCBs in situ.

Dataset Information

Electrical stimulation improves microbial salinity resistance and organofluorine removal in bioelectrochemical systems.

Publications

Electrical stimulation improves microbial salinity resistance and organofluorine removal in bioelectrochemical systems.

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