Reverse genetics system for studying human rhinovirus infections.
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ABSTRACT:
SUBMITTER: Lee WM
PROVIDER: S-EPMC4422385 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Reverse genetics system for studying human rhinovirus infections.
Lee Wai-Ming WM Wang Wensheng W Bochkov Yury A YA Lee Iris I
Methods in molecular biology (Clifton, N.J.) 20150101
Human rhinovirus (HRV) contains a 7.2 kb messenger-sense RNA genome which is the template for reproducing progeny viruses after it enters the cytoplasm of a host cell. Reverse genetics refers to the regeneration of progeny viruses from an artificial cDNA copy of the RNA genome of an RNA virus. It has been a powerful molecular genetic tool for studying HRV and other RNA viruses because the artificial DNA stage makes it practical to introduce specific mutations into the viral RNA genome. This chap ...[more]
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