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The Early Metazoan Trichoplax adhaerens Possesses a Functional O-GlcNAc System.

ABSTRACT: Protein O-GlcNAcylation is a reversible post-translational signaling modification of nucleocytoplasmic proteins that is essential for embryonic development in bilateria. In a search for a reductionist model to study O-GlcNAc signaling, we discovered the presence of functional O-GlcNAc transferase (OGT), O-GlcNAcase (OGA), and nucleocytoplasmic protein O-GlcNAcylation in the most basal extant animal, the placozoan Trichoplax adhaerens. We show via enzymatic characterization of Trichoplax OGT/OGA and genetic rescue experiments in Drosophila melanogaster that these proteins possess activities/functions similar to their bilaterian counterparts. The acquisition of O-GlcNAc signaling by metazoa may have facilitated the rapid and complex signaling mechanisms required for the evolution of multicellular organisms.

SUBMITTER: Selvan N

PROVIDER: S-EPMC4424335 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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The Early Metazoan Trichoplax adhaerens Possesses a Functional O-GlcNAc System.

Selvan Nithya N Mariappa Daniel D van den Toorn Henk W P HW Heck Albert J R AJ Ferenbach Andrew T AT van Aalten Daan M F DM

The Journal of biological chemistry 20150316 19

Protein O-GlcNAcylation is a reversible post-translational signaling modification of nucleocytoplasmic proteins that is essential for embryonic development in bilateria. In a search for a reductionist model to study O-GlcNAc signaling, we discovered the presence of functional O-GlcNAc transferase (OGT), O-GlcNAcase (OGA), and nucleocytoplasmic protein O-GlcNAcylation in the most basal extant animal, the placozoan Trichoplax adhaerens. We show via enzymatic characterization of Trichoplax OGT/OGA ...[more]

PMID: 25778404

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