Compaction, fusion, and functional activation of three-dimensional human mesenchymal stem cell aggregate.

ABSTRACT: Human mesenchymal stem cells (hMSCs) are primary candidates in cell therapy and tissue engineering and are being tested in clinical trials for a wide range of diseases. Originally isolated and expanded as plastic adherent cells, hMSCs have intriguing properties of in vitro self-assembly into three-dimensional (3D) aggregates that improve a range of biological properties, including multilineage potential, secretion of therapeutic factors, and resistance against ischemic condition. While cell-cell contacts and cell-extracellular matrix interactions mediate 3D cell aggregation, the adaptive changes of hMSC cytoskeleton during self-assembly and associated metabolic reconfiguration may also influence aggregate properties and functional activation. In this study, we investigated the role of acti

SUBMITTER: Tsai AC 

PROVIDER: S-EPMC4426301 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature