Predictive polymer modeling reveals coupled fluctuations in chromosome conformation and transcription.

ABSTRACT: A new level of chromosome organization, topologically associating domains (TADs), was recently uncovered by chromosome conformation capture (3C) techniques. To explore TAD structure and function, we developed a polymer model that can extract the full repertoire of chromatin conformations within TADs from population-based 3C data. This model predicts actual physical distances and to what extent chromosomal contacts vary between cells. It also identifies interactions within single TADs that stabilize boundaries between TADs and allows us to identify and genetically validate key structural elements within TADs. Combining the model's predictions with high-resolution DNA FISH and quantitative RNA FISH for TADs within the X-inactivation center (Xic), we dissect the relationship between transcrip

SUBMITTER: Giorgetti L 

PROVIDER: S-EPMC4427251 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature