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High-speed label-free functional photoacoustic microscopy of mouse brain in action.

ABSTRACT: We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

SUBMITTER: Yao J

PROVIDER: S-EPMC4428901 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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High-speed label-free functional photoacoustic microscopy of mouse brain in action.

Yao Junjie J Wang Lidai L Yang Joon-Mo JM Maslov Konstantin I KI Wong Terence T W TT Li Lei L Huang Chih-Hsien CH Zou Jun J Wang Lihong V LV

Nature methods 20150330 5

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse br ...[more]

PMID: 25822799

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