Influence of animal husbandry practices on void spot assay outcomes in C57BL/6J male mice.

ABSTRACT:

Aims

Mice are increasingly being used as models to investigate aspects of urinary dysfunction that humans with lower urinary tract symptoms (LUTS) experience. One method used to examine voiding function is the spontaneous void spot assay. The purpose of this study was to characterize and identify animal husbandry conditions that might confound results of the spontaneous void spot assay in male C57Bl/6J mice.

Methods

Mice were placed in cages lined with filter paper for 4 hr and urine was visualized with UV transillumination. Voiding parameters including urine spot number, spot size, total urine area, primary void area, corner and center voiding were quantified.

Results

Adult male mice void more frequently with advancing age and a subpopulation (5-10%) display a freque

SUBMITTER: Keil KP 

PROVIDER: S-EPMC4428995 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature