Differential Effects of Tra2ß Isoforms on HIV-1 RNA Processing and Expression.

ABSTRACT: Balanced processing of HIV-1 RNA is critical to virus replication and is regulated by host factors. In this report, we demonstrate that overexpression of either Tra2? or Tra2? results in a marked reduction in HIV-1 Gag/Env expression, an effect associated with changes in HIV-1 RNA accumulation, altered viral splice site usage, and a block to export of HIV-1 genomic RNA. A natural isoform of Tra2? (Tra2ß3), lacking the N-terminal RS domain, also suppressed HIV-1 expression but had different effects on viral RNA processing. The functional differences between the Tra2? isoforms were also observed in the context of another RNA substrate indicating that these factors have distinct functions within the cell. Finally, we demonstrate that Tra2ß depletion results in a selective reduction in HIV-1 Env expression as well as an increase in multiply spliced viral RNA. Together, the findings indicate that Tra2?/? can play important roles in regulating HIV-1 RNA metabolism and expression.

SUBMITTER: Platt C 

PROVIDER: S-EPMC4430212 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature