Ontology highlight
ABSTRACT:
SUBMITTER: Hilton IB
PROVIDER: S-EPMC4430400 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Hilton Isaac B IB D'Ippolito Anthony M AM Vockley Christopher M CM Thakore Pratiksha I PI Crawford Gregory E GE Reddy Timothy E TE Gersbach Charles A CA
Nature biotechnology 20150406 5
Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fused to the catalytic core of the human acetyltransferase p300. The fusion protein catalyzes acetylation of histone H3 lysine 27 at its target sites, leading to robust transcriptional ...[more]