Ontology highlight
ABSTRACT:
SUBMITTER: Sinha NK
PROVIDER: S-EPMC4433149 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Molecular cell 20150416 3
In previous studies we observed that the helicase domain of Drosophila Dicer-2 (dmDcr-2) governs substrate recognition and cleavage efficiency, and that dsRNA termini are key to this discrimination. We now provide a mechanistic basis for these observations. We show that discrimination of termini occurs during initial binding. Without ATP, dmDcr-2 binds 3' overhanging, but not blunt, termini. By contrast, with ATP, dmDcr-2 binds both types of termini, with highest-affinity binding observed with b ...[more]