Ontology highlight
ABSTRACT:
SUBMITTER: Welker NC
PROVIDER: S-EPMC3061311 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Molecular cell 20110301 5
The role of Dicer's helicase domain is enigmatic, but in vivo it is required for processing certain endogenous siRNA, but not miRNA. By using Caenorhabditis elegans extracts or purified Drosophila Dicer-2 we compared activities of wild-type enzymes and those containing mutations in the helicase domain. We found the helicase domain was essential for cleaving dsRNA with blunt or 5'-overhanging termini, but not those with 3' overhangs, as found on miRNA precursors. Further, blunt termini, but not 3 ...[more]