Colorectal cancer severity and survival in correlation with tumour necrosis factor-alpha.

ABSTRACT: Colorectal cancer (CRC) development is strongly associated with innate immune mechanisms and intestinal inflammation. The aim of the study was to investigate the pre-operative serum levels of TNF-? and its correlation with cancer progression and survival in CRC patients taking into account the genotype of -308G/A promoter polymorphism in TNF-? gene (rs1800629). TNF-? -308G/A genotypes of 119 CRC cases and 177 no CRC controls were determined by restriction fragment length polymorphism assay (RFLP-PCR). TNF-? serum levels were measured by enzyme-linked immunosorbent assay (ELISA). Although no significant differences in allele and genotype frequencies between CRC and controls were observed, it should be noted that the minor allele-A and its homozygous genotype were overrepresented among CRC. In addition, allele-A was more frequent in early CRC patients compared to advanced cases. TNF-? serum level was significantly higher in CRC patients than in controls (36.1 ± 8.4 pg/mL vs. 18.66 ± 11 pg/mL; p = 0.0000001). In the subgroup analysis by tumour-node-metastasis stages, the highest TNF-? level was found in stage IV (42.7 ± 12.5 pg/mL) and was significantly elevated compared to earlier stages of CRC and controls. The survival rate of CRC patients with low TNF-? serum level, estimated as median survival, was significantly higher than that of patients with high levels of TNF-? (38.4 vs. 7.761 months; log rank test p = 0.00015) In conclusion, we can affirm that TNF-? affects tumour development along with disease progression which has an impact on the survival of CRC.

SUBMITTER: Stanilov N 

PROVIDER: S-EPMC4433886 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature