Ontology highlight
ABSTRACT:
SUBMITTER: Subach FV
PROVIDER: S-EPMC4439098 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Subach Fedor V FV Piatkevich Kiryl D KD Verkhusha Vladislav V VV
Nature methods 20111129 12
Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins. We discuss possibilities for knowledge-based rational design based on the photochemistry of fluorescent proteins and the position of the chromophore in protein structure. We consider advanc ...[more]