Ontology highlight
ABSTRACT:
SUBMITTER: Hribar KC
PROVIDER: S-EPMC4439309 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Hribar K C KC Finlay D D Ma X X Qu X X Ondeck M G MG Chung P H PH Zanella F F Engler A J AJ Sheikh F F Vuori K K Chen S C SC
Lab on a chip 20150422 11
Long-term culture and monitoring of individual multicellular spheroids and embryoid bodies (EBs) remains a challenge for in vitro cell propagation. Here, we used a continuous 3D projection printing approach - with an important modification of nonlinear exposure - to generate concave hydrogel microstructures that permit spheroid growth and long-term maintenance, without the need for spheroid transfer. Breast cancer spheroids grown to 10 d in the concave structures showed hypoxic cores and signs o ...[more]