Constraints contributed by chromatin looping limit recombination targeting during Ig class switch recombination.

ABSTRACT: Engagement of promoters with distal elements in long-range looping interactions has been implicated in regulation of Ig class switch recombination (CSR). The principles determining the spatial and regulatory relationships among Igh transcriptional elements remain poorly defined. We examined the chromosome conformation of C region (CH) loci that are targeted for CSR in a cytokine-dependent fashion in mature B lymphocytes. Germline transcription (GLT) of the ?1 and ? CH loci is controlled by two transcription factors, IL-4-inducible STAT6 and LPS-activated NF-?B. We showed that although STAT6 deficiency triggered loss of GLT, deletion of NF-?B p50 abolished both GLT and ?1 locus:enhancer looping. Thus, chromatin looping between CH loci and Igh enhancers is independent of GLT production and STAT6, whereas the establishment and maintenance of these chromatin contacts requires NF-?B p50. Comparative analysis of the endogenous ?1 locus and a knock-in heterologous promoter in mice identified the promoter per se as the interactive looping element and showed that transcription elongation is dispensable for promoter/enhancer interactions. Interposition of the LPS-responsive heterologous promoter between the LPS-inducible ?3 and ?2b loci altered GLT expression and essentially abolished direct IgG2b switching while maintaining a sequential ???3??2b format. Our study provides evidence that promoter/enhancer looping interactions can introduce negative constraints on distal promoters and affect their ability to engage in germline transcription and determine CSR targeting.

SUBMITTER: Feldman S 

PROVIDER: S-EPMC4440573 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature