Dataset Information

First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon.

ABSTRACT: Pneumocystis colonization may play a role in transmission and local inflammatory response. It was explored in patients with respiratory diseases in North Lebanon. Overall prevalence reached only 5.2% (95% CI 2.13-10.47) but it was higher (17.3%) in the subpopulation of patients with chronic obstructive pulmonary disease (COPD). COPD was the only factor associated with a significantly increased risk of colonization. mtLSU genotyping revealed predominance of genotype 2, identified in five patients (71.4%), including one patient who had co-infection with genotype 3. These first data in North Lebanon confirm Pneumocystis circulation among patients with respiratory diseases and the potential for transmission to immunocompromised patients.

SUBMITTER: Khalife S

PROVIDER: S-EPMC4442690 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon.

Khalife S S Aliouat E M EM Aliouat E M EM Aliouat-Denis C M CM Gantois N N Devos P P Mallat H H Dei-Cas E E Dabboussi F F Hamze M M Fréalle E E

New microbes and new infections 20150304

Pneumocystis colonization may play a role in transmission and local inflammatory response. It was explored in patients with respiratory diseases in North Lebanon. Overall prevalence reached only 5.2% (95% CI 2.13-10.47) but it was higher (17.3%) in the subpopulation of patients with chronic obstructive pulmonary disease (COPD). COPD was the only factor associated with a significantly increased risk of colonization. mtLSU genotyping revealed predominance of genotype 2, identified in five patients ...[more]

PMID: 26042187

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Project description:BACKGROUND:Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungal pathogen and the role of its colonization in pulmonary diseases has become a popular focus in recent years. The aim of this study was to develop a modified loop-mediated isothermal amplification (LAMP) assay for detection of Pneumocystis jirovecii (P. jirovecii) DNA amongst non-HIV patients with various pulmonary diseases and use it to examine the prevalence and assess the association of P. jirovecii colonization with clinical characteristics of these diseases. METHODS:We modified the previously reported LAMP assay for P. jirovecii by adding real-time detection. This method was used to detect P. jirovecii colonization in pulmonary samples collected from 403 non-HIV patients with various pulmonary diseases enrolled from 5 hospitals in China. We determined the prevalence of P. jirovecii colonization in 7 types of pulmonary diseases and assessed the association of P. jirovecii colonization with clinical characteristics of these diseases. RESULTS:The modified LAMP assay showed no cross-reactivity with other common pulmonary microbes and was 1000 times more sensitive than that of conventional PCR. Using the modified LAMP assay, we detected P. jirovecii colonization in 281 (69.7%) of the 403 patients enrolled. P. jirovecii colonization was more common in interstitial lung diseases than in chronic obstructive pulmonary disease (COPD) (84.6% vs 64.5%, P?<?0.05). Patients with acute exacerbation of COPD had a higher prevalence of P. jirovecii colonization compared to patients with stabilized COPD (67.4% vs 43.3%, P?<?0.05). P. jirovecii colonization was associated with decreased pulmonary function, increased levels of 1,3-?-D-glucan and C-reactive protein, and decreased levels of CD4+ T-cell counts (P?<?0.05 for each). Approximately 70% of P. jirovecii colonized patients had confections with other fungi or bacteria. CONCLUSIONS:We developed a modified LAMP assay for detecting P. jirovecii. Our multi-center study of 403 patients supports that P. jirovecii colonization is a risk factor for the development of pulmonary diseases and highlights the need to further study the pathogenesis and transmission of P. jirovecii colonization in pulmonary diseases.

Dataset Information

First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon.

Publications

First data on Pneumocystis jirovecii colonization in patients with respiratory diseases in North Lebanon.

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