Unknown

Dataset Information

0

IgE Reactivity of the Dog Lipocalin Allergen Can f 4 and the Development of a Sandwich ELISA for Its Quantification.


ABSTRACT: PURPOSE:Divergent results on the IgE reactivity of dog-allergic subjects to Can f 4 have been reported. The aim of this study was to evaluate the significance of Can f 4 in dog allergy and to develop an immunochemical method for measuring Can f 4 content in environmental samples. METHODS:We purified the natural dog allergen Can f 4 from a dog dander extract by monoclonal antibody-based affinity chromatography and generated its variant in a recombinant form. Sixty-three dog-allergic patients and 12 nonallergic control subjects were recruited in the study. The IgE-binding capacity of natural Can f 4 and its recombinant variant was assessed by ELISA, immunoblotting, and skin prick tests (SPT). RESULTS:Eighty-one percent of the dog-allergic patients showed a positive result to the immunoaffinity-purified natural Can f 4 in IgE ELISA, but only 46% in IgE immunoblotting. Respective results with the recombinant Can f 4 variant were 54% and 49%. SPT results reflected those obtained in ELISA and immunoblotting. The overall IgE reactivity of the immunoaffinity-purified natural Can f 4 was found to depend strongly on the integrity of the allergen's conformation. A sandwich ELISA based on monoclonal antibodies was found to be functional for measuring Can f 4 in environmental samples. CONCLUSIONS:Can f 4 is a major allergen of dog together with Can f 1 and Can f 5. In combination with other dog allergens, it improves the reliability of allergy tests in dog allergy.

SUBMITTER: Rytkonen-Nissinen M 

PROVIDER: S-EPMC4446637 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

IgE Reactivity of the Dog Lipocalin Allergen Can f 4 and the Development of a Sandwich ELISA for Its Quantification.

Rytkönen-Nissinen Marja M   Saarelainen Soili S   Randell Jukka J   Häyrinen Jukka J   Kalkkinen Nisse N   Virtanen Tuomas T  

Allergy, asthma & immunology research 20150305 4


<h4>Purpose</h4>Divergent results on the IgE reactivity of dog-allergic subjects to Can f 4 have been reported. The aim of this study was to evaluate the significance of Can f 4 in dog allergy and to develop an immunochemical method for measuring Can f 4 content in environmental samples.<h4>Methods</h4>We purified the natural dog allergen Can f 4 from a dog dander extract by monoclonal antibody-based affinity chromatography and generated its variant in a recombinant form. Sixty-three dog-allergi  ...[more]

Similar Datasets

| S-EPMC7610822 | biostudies-literature
| S-EPMC5610169 | biostudies-literature
| S-EPMC6757120 | biostudies-literature
| S-EPMC6816904 | biostudies-literature
| S-EPMC6746357 | biostudies-literature
| S-EPMC4218792 | biostudies-literature
| S-EPMC5014315 | biostudies-literature
| S-EPMC3072074 | biostudies-literature
| S-EPMC3574828 | biostudies-other
| S-EPMC9334453 | biostudies-literature