Ontology highlight
ABSTRACT:
SUBMITTER: Yi T
PROVIDER: S-EPMC4448125 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Yi Tingfang T Arthanari Haribabu H Akabayov Barak B Song Huaidong H Papadopoulos Evangelos E Qi Hank H HH Jedrychowski Mark M Güttler Thomas T Guo Cuicui C Luna Rafael E RE Gygi Steven P SP Huang Stephen A SA Wagner Gerhard G
Nature communications 20150528
MicroRNA (miRNA) biogenesis and miRNA-guided RNA interference (RNAi) are essential for gene expression in eukaryotes. Here we report that translation initiation factor eIF1A directly interacts with Ago2 and promotes Ago2 activities in RNAi and miR-451 biogenesis. Biochemical and NMR analyses demonstrate that eIF1A binds to the MID domain of Ago2 and this interaction does not impair translation initiation. Alanine mutation of the Ago2-facing Lys56 in eIF1A impairs RNAi activities in human cells a ...[more]