Ontology highlight
ABSTRACT:
SUBMITTER: Warsen AE
PROVIDER: S-EPMC444826 | biostudies-literature | 2004 Jul
REPOSITORIES: biostudies-literature
Warsen Adelaide E AE Krug Melissa J MJ LaFrentz Stacey S Stanek Danielle R DR Loge Frank J FJ Call Douglas R DR
Applied and environmental microbiology 20040701 7
We developed a DNA microarray suitable for simultaneous detection and discrimination between multiple bacterial species based on 16S ribosomal DNA (rDNA) polymorphisms using glass slides. Microarray probes (22- to 31-mer oligonucleotides) were spotted onto Teflon-masked, epoxy-silane-derivatized glass slides using a robotic arrayer. PCR products (ca. 199 bp) were generated using biotinylated, universal primer sequences, and these products were hybridized overnight (55 degrees C) to the microarra ...[more]