Unknown

Dataset Information

0

IL-33 attenuates the development of experimental autoimmune uveitis.


ABSTRACT: Interleukin-33 (IL-33) is associated with several important immune-mediated disorders. However, its role in uveitis, an important eye inflammatory disease, is unknown. Here, we investigated the function of IL-33 in the development of experimental autoimmune uveitis (EAU). IL-33 and IL-33 receptor (ST2) were expressed in murine retinal pigment epithelial (RPE) cells in culture, and IL-33 increased the expression of Il33 and Mcp1 mRNA in RPE cells. In situ, IL-33 was highly expressed in the inner nuclear cells of the retina of naïve mice, and its expression was elevated in EAU mice. ST2-deficient mice developed exacerbated EAU compared with WT mice, and administration of IL-33 to WT mice significantly reduced EAU severity. The attenuated EAU in IL-33-treated mice was accompanied by decreased frequency of IFN-?+ and IL-17(+) CD4+ T cells and reduced IFN-? and IL-17 production but with increased frequency of IL-5(+) and IL-4(+) CD4 T cells and IL-5 production in the draining lymph node and spleen. Macrophages from the IL-33-treated mice show a significantly higher polarization toward an alternatively activated macrophage phenotype. Our results therefore demonstrate that the endogenous IL-33/ST2 pathway plays an important role in EAU, and suggest that IL-33 represents a potential option for treatment of uveitis.

SUBMITTER: Barbour M 

PROVIDER: S-EPMC4449115 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications


Interleukin-33 (IL-33) is associated with several important immune-mediated disorders. However, its role in uveitis, an important eye inflammatory disease, is unknown. Here, we investigated the function of IL-33 in the development of experimental autoimmune uveitis (EAU). IL-33 and IL-33 receptor (ST2) were expressed in murine retinal pigment epithelial (RPE) cells in culture, and IL-33 increased the expression of Il33 and Mcp1 mRNA in RPE cells. In situ, IL-33 was highly expressed in the inner  ...[more]

Similar Datasets

| S-EPMC3111309 | biostudies-literature
| S-EPMC3344911 | biostudies-literature
| S-EPMC9050930 | biostudies-literature
| S-EPMC3392008 | biostudies-literature
2022-09-10 | GSE213034 | GEO
| S-EPMC5053150 | biostudies-literature
| S-EPMC8305313 | biostudies-literature
| S-EPMC4707108 | biostudies-literature
| S-EPMC3498374 | biostudies-literature
| S-EPMC8027130 | biostudies-literature