Culture of East Indian sandalwood tree somatic embryos in air-lift bioreactors for production of santalols, phenolics and arabinogalactan proteins

ABSTRACT: The Indian Sandalwood tree is globally acclaimed for the precious essential oil and heartwood. Over-exploitation, diseases, and habitat loss have posed significant challenges to find an alternative bioresource for biomass production. Here, we report the successful growth of in vitro grown somatic embryos in 10?L air-lift bioreactors. Additionally, we characterized arabinogalactan proteins and small molecule constituents such as phenolics and terpenoids that are secreted by the suspended somatic embryos into the culture media. In parallel to the biochemical characterisation, we followed the entire developmental progression of proembryogenic masses into matured cotyledonary embryos during a single run of the bioreactor. The East Indian sandalwood tree, Santalum album, yields one of the costliest heartwoods and precious essential oil. Unsurprisingly, this endangered forest species is severely affected due to unmet global demands, illegal trade and harvesting, overharvesting and an epidemic mycoplasmal spike disease. In vitro micropropagation endeavours have resulted in defined in vitro stages such as somatic embryos that are amenable to mass production in bioreactors. We report on somatic embryo production in a 10-L air-lift-type bioreactor, and compare the growth and biochemical parameters with those of a 2-L air-lift-type bioreactor. For the 10-L bioreactor with biomass (475.7 ± 18 g fresh weight; P < 0.01), concomitantly santalols (5.2 ± 0.15 mg L?1; P < 0.05), phenolics (31 ± 1.6 mg L?1) and arabinogalactan proteins (AGPs) (39 ± 3.1 mg L?1; P < 0.05) are produced in 28 days. In addition, we identified and quantified several santalols and phenolics by means of high-performance thin-layer chromatography and reverse-phase high-pressure liquid chromatography analyses, respectively. Results indicate that 10-L-capacity air-lift bioreactors are capable of supporting somatic embryo cultures, while the extracellular medium provides opportunities for production of industrial raw materials such as santalols, phenolics and AGPs. This will prove useful for further optimization and scale-up studies of plant-produced metabolites.

SUBMITTER: Misra B 

PROVIDER: S-EPMC4455360 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature