Ontology highlight
ABSTRACT:
SUBMITTER: Lee JS
PROVIDER: S-EPMC4455777 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Lee Jeong-Soo JS Kwak Su-Jin SJ Kim Jungeun J Kim Ae-Kyeong AK Noh Hae Min HM Kim Jin-Soo JS Yu Kweon K
G3 (Bethesda, Md.) 20140528 7
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay. ...[more]