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In vivo imaging of CREB phosphorylation in awake-mouse brain.

ABSTRACT: The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is required for elucidating the mechanism of memory consolidation, imaging of phosphorylation of a particular protein in the brain of live animals is impossible at present. Here, we developed a method for visualizing the CREB phosphorylation in the cerebral cortex of an awake mouse using a split luciferase technique. Using this technique, we demonstrated the correlation between the change in CREB phosphorylation at a particular region in the brain and behavioral consequences induced by the administration of reserpine, a psychotropic agent.

SUBMITTER: Ishimoto T

PROVIDER: S-EPMC4456726 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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In vivo imaging of CREB phosphorylation in awake-mouse brain.

Ishimoto Tetsuya T Mano Hiroki H Mori Hisashi H

Scientific reports 20150605

The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is required for elucidating the mechanism of memory consolidation, imaging of phosphorylation of a particular protein in the brain of live animals is impossible at present. Here, we developed a method for visua ...[more]

PMID: 26044058

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