Kilohertz two-photon brain imaging in awake mice.
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ABSTRACT:
SUBMITTER: Zhang T
PROVIDER: S-EPMC9438750 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Kilohertz two-photon brain imaging in awake mice.
Zhang Tong T Hernandez Oscar O Chrapkiewicz Radosław R Shai Adam A Wagner Mark J MJ Zhang Yanping Y Wu Cheng-Hsun CH Li Jin Zhong JZ Inoue Masatoshi M Gong Yiyang Y Ahanonu Biafra B Zeng Hongkui H Bito Haruhiko H Schnitzer Mark J MJ
Nature methods 20191028 11
Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10-30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000-211,000 µm<sup>2</sup> areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca<sup>2+</sup> spiking with millisecond-scale timing resolution in the brains of awak ...[more]
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