Ontology highlight
ABSTRACT:
SUBMITTER: Bratanov D
PROVIDER: S-EPMC4457421 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
PloS one 20150605 6
Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allows one to localize the underlying problem of poor expression of a membrane protein using bR as an example. Our approach is based on constructing chimeric proteins comprising parts of a protein of inte ...[more]