An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood.
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ABSTRACT:
SUBMITTER: Li S
PROVIDER: S-EPMC4458728 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
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An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood.
Li Siyang S Plouffe Brian D BD Belov Arseniy M AM Ray Somak S Wang Xianzhe X Murthy Shashi K SK Karger Barry L BL Ivanov Alexander R AR
Molecular & cellular proteomics : MCP 20150309 6
Isolation and molecular characterization of rare cells (e.g. circulating tumor and stem cells) within biological fluids and tissues has significant potential in clinical diagnostics and personalized medicine. The present work describes an integrated platform of sample procurement, preparation, and analysis for deep proteomic profiling of rare cells in blood. Microfluidic magnetophoretic isolation of target cells spiked into 1 ml of blood at the level of 1000-2000 cells/ml, followed by focused ac ...[more]
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