Continuous enhancement of iturin A production by Bacillus subtilis with a stepwise two-stage glucose feeding strategy.

ABSTRACT:

Background

The lipopeptide antibiotic iturin A is an attractive biopesticide with the potential to replace chemical-based pesticides for controlling plant pathogens. However, its industrial fermentation has not been realized due to the high production costs and low product concentrations. This study aims to enhance iturin A production by performing a novel fermentation process with effective glucose feeding control using rapeseed meal as a low-cost nitrogen source.

Results

We demonstrated that continuous and significant enhancement of iturin A production could be achieved by a novel two-stage glucose-feeding strategy with a stepwise decrease in feeding rate. Using this strategy, the ratio of spores to total cells could be maintained at a desirable/stable level of 0.80-0.86,

SUBMITTER: Jin H 

PROVIDER: S-EPMC4460961 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature