Ontology highlight
ABSTRACT:
SUBMITTER: Park J
PROVIDER: S-EPMC4461349 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Park Jimin J Lee Daeun D Kim Mi Sun MS Kim Dae Yong DY Shin Dong Hae DH
Acta crystallographica. Section F, Structural biology communications 20150522 Pt 6
3-Deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI), the third enzyme in the pathway for the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid (KDO), hydrolyzes KDO 8-phosphate to KDO and inorganic phosphate. YrbI belongs to the haloacid dehalogenase (HAD) superfamily, which is a large family of magnesium-dependent phosphatase/phosphotransferase enzymes. In this study, YrbI from Burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified a ...[more]