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Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.

ABSTRACT: Using a G-quadruplex bait, we identified the transcription co-activator Sub1 as a G-quadruplex binding protein by quantitative LC-MS/MS and demonstrated in vivo G-quadruplex binding by ChIP. In vitro, Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes. This provides a possible mechanism by which G-quadruplexes can influence gene transcription.

SUBMITTER: Gao J

PROVIDER: S-EPMC4465349 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.

Gao Jun J Zybailov Boris L BL Byrd Alicia K AK Griffin Wezley C WC Chib Shubeena S Mackintosh Samuel G SG Tackett Alan J AJ Raney Kevin D KD

Chemical communications (Cambridge, England) 20150401 33

Using a G-quadruplex bait, we identified the transcription co-activator Sub1 as a G-quadruplex binding protein by quantitative LC-MS/MS and demonstrated in vivo G-quadruplex binding by ChIP. In vitro, Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes. This provides a possible mechanism by which G-quadruplexes can influence gene transcription. ...[more]

PMID: 25813861

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