Dataset Information

Scan-Free Absorbance Spectral Imaging A(x, y, ?) of Single Live Algal Cells for Quantifying Absorbance of Cell Suspensions.

ABSTRACT: Label-free, non-invasive, rapid absorbance spectral imaging A(x,y,?) microscopy of single live cells at 1.2 ?m × 1.2 ?m resolution with an NA = 0.85 objective was developed and applied to unicellular green algae Chlamydomonas reinhardtii. By introducing the fiber assembly to rearrange a two-dimensional image to the one-dimensional array to fit the slit of an imaging spectrograph equipped with a CCD detector, scan-free acquisition of three-dimensional information of A(x,y,?) was realized. The space-resolved absorbance spectra of the eyespot, an orange organelle about 1 ?m, were extracted from the green-color background in a chlorophyll-rich single live cell absorbance image. Characteristic absorbance change in the cell suspension after hydrogen photoproduction in C. reinhardtii was investigated to find a single 715-nm absorption peak was locally distributed within single cells. The formula to calculate the absorbance of cell suspensions from that of single cells was presented to obtain a quantitative, parameter-free agreement with the experiment. It is quantitatively shown that the average number of chlorophylls per cell is significantly underestimated when it is evaluated from the absorbance of the cell suspensions due to the package effect.

SUBMITTER: Isono T

PROVIDER: S-EPMC4465668 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Scan-Free Absorbance Spectral Imaging A(x, y, λ) of Single Live Algal Cells for Quantifying Absorbance of Cell Suspensions.

Isono Takumi T Yamashita Kyohei K Momose Daisuke D Kobayashi Hiroki H Kitamura Masashi M Nishiyama Yusuke Y Hosoya Takahiro T Kanda Hiroaki H Kudo Ayane A Okada Norihide N Yagi Takafumi T Nakata Kazuaki K Mineki Shigeru S Tokunaga Eiji E

PloS one 20150610 6

Label-free, non-invasive, rapid absorbance spectral imaging A(x,y,λ) microscopy of single live cells at 1.2 μm × 1.2 μm resolution with an NA = 0.85 objective was developed and applied to unicellular green algae Chlamydomonas reinhardtii. By introducing the fiber assembly to rearrange a two-dimensional image to the one-dimensional array to fit the slit of an imaging spectrograph equipped with a CCD detector, scan-free acquisition of three-dimensional information of A(x,y,λ) was realized. The spa ...[more]

PMID: 26061268

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Project description:Plants commonly respond to UV radiation through the accumulation of flavonoids and related phenolic compounds which potentially ameliorate UV-damage to crucial internal structures. However, the seasonal dynamics of leaf flavonoids corresponding to epidermal UV absorbance is highly variable in nature, and it remains uncertain how environmental factors combine to govern flavonoid accumulation and degradation. We studied leaf UV-A absorbance of species composing the understorey plant community throughout two growing seasons under five adjacent tree canopies in southern Finland. We compared the relationship between leaf flavonol index (Iflav-repeatedly measured with an optical leaf clip Dualex) and measured spectral irradiance, understorey and canopy phenology, air temperature and snowpack variables, whole leaf flavonoid extracts, and leaf age. Strong seasonal patterns and stand-related differences were apparent in Iflav of both understorey plant communities and individual species, including divergent trends in Iflav during spring and autumn. Comparing the heterogeneity of the understorey light environment and its spectral composition in looking for potential drivers of seasonal changes in Iflav, we found that unweighted UV-A irradiance, or the effective UV dose calculated according to the biological spectral weighting function (BSWF) for plant growth (PG action spectrum), in understorey shade had a strong relationship with Iflav. Furthermore, understorey species seemed to adjust Iflav to low background diffuse irradiance rather than infrequent high direct-beam irradiance in sunflecks during summer, since leaves produced during or after canopy closure had low Iflav. In conclusion, we found the level of epidermal flavonoids in forest understorey species to be plastic, adjusting to climatic conditions, and differing according to species' leaf retention strategy and new leaf production, all of which contribute to the seasonal trends in leaf flavonoids found within forest stands.

Dataset Information

Scan-Free Absorbance Spectral Imaging A(x, y, ?) of Single Live Algal Cells for Quantifying Absorbance of Cell Suspensions.

Publications

Scan-Free Absorbance Spectral Imaging A(x, y, λ) of Single Live Algal Cells for Quantifying Absorbance of Cell Suspensions.

Similar Datasets

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