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Microfluidic synthesis of rigid nanovesicles for hydrophilic reagents delivery.

ABSTRACT: We present a hollow-structured rigid nanovesicle (RNV) fabricated by a multi-stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic-co-glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) simulations. The entrapment efficiency of hydrophilic reagents such as calcein, rhodamine?B and siRNA inside the hollow water core of RNV is ?90?%. In comparison with the combination of free Dox and siRNA, RNV that co-encapsulate siRNA and doxorubicin (Dox) reveals a significantly enhanced anti-tumor effect for a multi-drug resistant tumor model.

SUBMITTER: Zhang L

PROVIDER: S-EPMC4471572 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Microfluidic synthesis of rigid nanovesicles for hydrophilic reagents delivery.

Zhang Lu L Feng Qiang Q Wang Jiuling J Sun Jiashu J Shi Xinghua X Jiang Xingyu X

Angewandte Chemie (International ed. in English) 20150220 13

We present a hollow-structured rigid nanovesicle (RNV) fabricated by a multi-stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic-co-glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) ...[more]

PMID: 25704675

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