Ontology highlight
ABSTRACT:
SUBMITTER: Cottee MA
PROVIDER: S-EPMC4471874 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Cottee Matthew A MA Muschalik Nadine N Johnson Steven S Leveson Joanna J Raff Jordan W JW Lea Susan M SM
eLife 20150523
Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the s ...[more]