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Imaging of tumor clones with differential liver colonization.

ABSTRACT: We present a model of hepatic colorectal metastases which represents monoclonal cell lines double-labeled by luciferase and tdTomato. These cells form liver metastasis in varying numbers and patterns similar to those observed in patients. Using in vivo and ex vivo luminescent and fluorescent imaging we determine the growth kinetics and clonogenic frequency of tumor cells colonizing liver. Molecular profiling detected stable expressional differences between clones consistent with their phenotypes. The data indicate that clinically relevant phenotypes of liver metastases can be modeled in vivo.

SUBMITTER: Oshima G

PROVIDER: S-EPMC4476146 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Imaging of tumor clones with differential liver colonization.

Oshima Go G Wightman Sean C SC Uppal Abhineet A Stack Melinda E ME Pitroda Sean P SP Oskvarek Jonathan J JJ Huang Xiaona X Posner Mitchell C MC Hellman Samuel S Weichselbaum Ralph R RR Khodarev Nikolai N NN

Scientific reports 20150622

We present a model of hepatic colorectal metastases which represents monoclonal cell lines double-labeled by luciferase and tdTomato. These cells form liver metastasis in varying numbers and patterns similar to those observed in patients. Using in vivo and ex vivo luminescent and fluorescent imaging we determine the growth kinetics and clonogenic frequency of tumor cells colonizing liver. Molecular profiling detected stable expressional differences between clones consistent with their phenotypes ...[more]

PMID: 26094901

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