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Chemical synthesis of the outer core oligosaccharide of Escherichia coli R3 and immunological evaluation.

ABSTRACT: Lipopolysaccharides (LPS), major virulence determinants in Gram-negative bacteria, are responsible for many pathophysiological responses and can elicit strong immune responses. In order to better understand the role of LPS in host-pathogen interactions and elucidate the immunogenic properties of LPS outer core oligosaccharides, an all ?-linked Escherichia coli R3 outer core pentasaccharide was first synthesized with a propyl amino linker at the reducing end. This oligosaccharide was also covalently conjugated to a carrier protein (CRM197) via the reducing end propyl amino linker. Immunological analysis demonstrated that this glycoconjugate can elicit specific anti-pentasaccharide antibodies with in vitro bactericidal activity. These findings will contribute to the further exploration of this pentasaccharide antigen as a vaccine candidate.

SUBMITTER: Shang W

PROVIDER: S-EPMC4481194 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Chemical synthesis of the outer core oligosaccharide of Escherichia coli R3 and immunological evaluation.

Shang Wenjing W Xiao Zhongying Z Yu Zaikuan Z Wei Na N Zhao Guohui G Zhang Qing Q Wei Mohui M Wang Xuan X Wang Peng George PG Li Tiehai T

Organic & biomolecular chemistry 20150312 14

Lipopolysaccharides (LPS), major virulence determinants in Gram-negative bacteria, are responsible for many pathophysiological responses and can elicit strong immune responses. In order to better understand the role of LPS in host-pathogen interactions and elucidate the immunogenic properties of LPS outer core oligosaccharides, an all α-linked Escherichia coli R3 outer core pentasaccharide was first synthesized with a propyl amino linker at the reducing end. This oligosaccharide was also covalen ...[more]

PMID: 25764373

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